Enzyme; Fitness landscape; Microfluidics; Catalytic properties

Evolutionary-Scale Enzymology Enables Biochemical Constant Prediction Across a Multi-Peaked Catalytic Landscape

Evolutionary-Scale Enzymology Enables Biochemical Constant Prediction Across a Multi-Peaked Catalytic Landscape

Duncan F. Muir, Garrison P. R. Asper, Pascal Notin, Jacob A. Posner, Debora S. Marks, Michael J. Keiser, Margaux M. Pinney
Preprint. A microfluidic platform to measure catalytic constants for hundreds of Adenylate Kinase variants, enabling mapping of the sequence-catalysis landscape and development of superior predictive models.
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